Not exactly. The paired batteries need to be about the same age / cycles. As a battery ages, it will loose C-rating and capacity. So if you have one old well used battery, and match it up with a fresh new pack, the old pack will get worked harder and dump sooner. The end result will be that the old pack will quickly be killed.
If one of a paired set of 2s packs dies, it is best to retire the other pack to 2s only duty, and buy another pair of 2s packs (or a 4s brick).
True in theory, but in practice, this would only matter so someone trying to / NEEDING TO get every last bit of energy out of a battery. For us bashers, if we assume your ESC/Batteries have proper sized wiring (10/12 gauge) and good plugs (XT90s are good) the extra 0.5%-2% you would gain by clipping out 2"-4" of wire and one plug won't be noticed. Plus, if you go to an on-board plug pack or use something like the
XT150's, you don't have the extra plug, so no issue.
If you are building a speed run car, and already running +130mph, and trying to get that extra 1mph, then looking to wire and plugs might gain you some of that. Might.
Jerry